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In prokaryotic gene expression, regulation is essential to conserve energy and adapt to environmental changes. Gene expression is primarily controlled at the transcriptional level through positive and negative control mechanisms, which involve regulatory proteins interacting with DNA sequences near the promoter to modulate RNA polymerase activity. Negative Control Mechanisms: Negative control involves a repressor protein that binds to an operator sequence or regulatory binding site to block transcription. The repressor prevents RNA polymerase from transcribing the downstream structural genes. Negative control can operate in two main ways: ____ _________ _______ __________ _________ _________ __________ ___ ___ __________.
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