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Isozymes, also known as isoenzymes, are multiple molecular forms of an enzyme that catalyze the same biochemical reaction but differ in their physicochemical properties such as structure, kinetic behavior, electrophoretic mobility, and regulatory characteristics. The origin of isozymes is closely linked to genetic, evolutionary, and cellular mechanisms that allow organisms to fine-tune metabolic processes according to tissue type, developmental stage, or environmental conditions. One major origin of isozymes is gene duplication followed by divergence. During evolution, a gene encoding an enzyme may be duplicated, and over time the duplicate genes accumulate mutations. While the core catalytic function is __________ __________ ____ _________ __________ ________ ________.
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