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Gene therapy relies on efficient delivery systems to introduce therapeutic genes into target cells, broadly classified into viral and non-viral vectors. These two systems differ significantly in structure, efficiency, safety, and scope of application, influencing their suitability for treating genetic and acquired disorders such as cystic fibrosis, hemophilia, cancer, and dermatological diseases. Viral vectors are derived from genetically modified viruses such as adenovirus, adeno-associated virus (AAV), retrovirus, and lentivirus, in which pathogenic genes are removed and replaced with therapeutic DNA. Their major advantage lies in high transfection efficiency, __________ _______ _____ ______ _________ _______ _________ _____ _______ _________ ___ ____.
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