Name two inhibitors of glycolysis and explain their mechanism of action.

Two important inhibitors of glycolysis are fluoride and arsenate. Both of these substances interfere with different steps of the glycolytic pathway, hindering energy production.

1. Fluoride: Fluoride acts as an inhibitor of glycolysis by inhibiting the enzyme enolase, which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. This inhibition results in the accumulation of 2-phosphoglycerate ______ _________ _________ _______ ________ ________ _____.
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