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The increased UV absorption of denatured DNA is primarily due to structural changes in the DNA molecule, which affect the interaction between its aromatic bases and incident ultraviolet light. DNA is composed of four nitrogenous bases—adenine, thymine, cytosine, and guanine—linked to a sugar-phosphate backbone. In its native double-helical form, these bases are stacked in a highly ordered manner through hydrophobic interactions and _______ _____ _________ ______ _____ ____ ________ ____ ______ _____ _________.
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