Describe the methods based on polarity of enzyme purification.

Enzyme purification is a crucial step in biotechnological and pharmaceutical applications, ensuring the isolation of enzymes in their purest form. One of the key methods used for purification is based on the polarity of enzymes. These methods exploit differences in hydrophobic and hydrophilic interactions to separate enzymes from other biomolecules.  

Hydrophobic Interaction Chromatography (HIC) is a widely used technique that leverages ____ ___ ______ __________ ____ ____ _________ ______ _________.
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