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Ammonium sulphate precipitation is a classical method used for the fractionation and purification of proteins based on their solubility differences. Proteins exhibit varying solubility in aqueous solutions depending on the ionic strength, pH, and temperature. Ammonium sulphate, being a highly soluble salt, is used to increase the ionic strength of the solution, which reduces the solubility of proteins and causes them to precipitate. This process is often referred to as "salting out." The underlying principle of ammonium sulphate precipitation relies on the competition between the salt ions ______ ___ ___ _____ _______ ______ ______.
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