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Tandem mass spectrometry, often abbreviated as MS/MS, is an advanced analytical technique used to analyze and identify molecules, particularly proteins and their components. The working principle of tandem mass spectrometry involves multiple stages of mass analysis separated by a fragmentation step, which allows for detailed structural information to be obtained from complex molecules. In protein analysis, MS/MS is particularly valuable for identifying peptide sequences, post-translational modifications, and protein-protein interactions. The process begins with ionization, where protein samples are first digested into smaller peptides, typically using enzymes like trypsin. These peptides __________ _____ _______ _________ ___.
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