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A protoplast is a plant cell that has had its cell wall removed, leaving behind the plasma membrane and the cell contents, including the nucleus, cytoplasm, and organelles. Protoplasts can be isolated from plant tissues through enzymatic digestion, which breaks down the cell wall components like cellulose and pectin. The protoplasts are important in plant biotechnology because they can be used in various genetic manipulation techniques, such as somatic hybridization.

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