Describe the technique of Agrobacterium-mediated transformation with t

Question

Describe the technique of Agrobacterium-mediated transformation with the help of a diagram.

23 Jan 2025

Answer :

Word Count : 629

Agrobacterium-mediated transformation is a widely used technique in genetic engineering, particularly for introducing foreign genes into plant cells. This process exploits the natural ability of the bacterium *Agrobacterium tumefaciens* to transfer a segment of its DNA into plant cells. The primary focus of this method lies in the *T-DNA* (Transfer DNA) from the bacterium’s plasmid, which is integrated into the plant genome. This method is commonly used in both agricultural biotechnology and economic botany for developing genetically modified plants with desirable traits such as pest resistance, herbicide tolerance, and improved nutritional content.

Mechanism of Agrobacterium-Mediated Transformation:

1. Identification of Target Plant Cells:
The transformation process begins by selecting a plant species that can be infected by *Agrobacterium tumefaciens*. The plant cells are typically in a proliferative state, such as in callus tissue or actively dividing cells.

2. Preparation of Agrobacterium:
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