Fluorescence can be used for qualitative as well as quantitative determination of the analyte. Discuss the factors affecting the quantitative applications of fluorescence. 

Fluorescence is a phenomenon where a molecule absorbs light energy and then emits light at a longer wavelength. It is a useful analytical technique for the detection and quantification of various analytes in different fields like medical, environmental, and pharmaceuticals. Fluorescence can be used for both qualitative and quantitative applications. However, the quantitative applications of fluorescence are affected by several factors that need to be taken into account to obtain accurate results. In this article, we will discuss the factors affecting the quantitative applications of fluorescence.

1. Excitation and Emission Spectra: The excitation and emission spectra of the fluorescent molecule are crucial factors affecting _______ ________ _____ __________ _______.
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