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The nuclear run-on assay is a molecular biology technique used to measure the transcriptional activity of specific genes by detecting newly synthesized RNA. This assay helps distinguish between transcriptional regulation and post-transcriptional regulation of gene expression, making it a valuable tool in genomics and proteomics studies. The procedure begins with the isolation of nuclei from cells under conditions that preserve ongoing transcription. Once isolated, the nuclei are _________ ____ _______ ________ ____.
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