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cDNA-AFLP (complementary DNA–Amplified Fragment Length Polymorphism) is a powerful transcript profiling technique used to analyze differential gene expression without prior sequence information. The principle is based on converting mRNA into cDNA, followed by restriction digestion, selective amplification, and separation of fragments to generate expression patterns. Initially, total RNA is isolated from the biological sample and mRNA is reverse transcribed into double-stranded cDNA using ___ __________ _________ ___ ______ _______ ________ ___.
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