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cDNA-AFLP (complementary DNA–Amplified Fragment Length Polymorphism) is a powerful transcript profiling technique used to analyze differential gene expression without prior sequence information. The principle is based on converting mRNA into cDNA, followed by restriction digestion, selective amplification, and separation of fragments to generate expression patterns. Initially, total RNA is isolated from the biological sample and mRNA is reverse transcribed into double-stranded cDNA using ________ ___ ___ __________ _____ _________ ________ _________ ___ ______ ____.
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