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RNA sequencing (RNA-Seq) is a powerful and widely used technique in genomics and proteomics for analyzing the transcriptome, which represents all RNA molecules, including mRNA, rRNA, tRNA, and non-coding RNAs, present in a cell at a given time. The principle of RNA-Seq is based on converting RNA into complementary DNA (cDNA), fragmenting it, and then sequencing these fragments using high-throughput sequencing technologies. This allows researchers to determine the sequence of RNA molecules, quantify gene expression levels, identify novel transcripts, alternative splicing events, and post-transcriptional modifications. RNA-Seq provides a high-resolution and comprehensive view of the transcriptome compared to traditional methods like microarrays, which are limited to detecting known _____ _______ ______ ____ _________ __________ _______ __________ ________ __________.
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