Word Count : 233

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Glutathione is a tripeptide composed of three amino acids: glutamate, cysteine, and glycine. Its synthesis occurs in two ATP-dependent steps primarily in the cytosol of plant cells. The first step is catalyzed by the enzyme γ-glutamylcysteine synthetase, which combines glutamate and cysteine to form γ-glutamylcysteine. This reaction is the rate-limiting step of glutathione synthesis and is tightly regulated _______ _____ _______ ___ ______.
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