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Subcellular fractionation is a technique used in cell biology to separate cellular components based on their size, density, and sedimentation properties. The process begins with the careful collection of cells from tissues or cultured cell lines. The cells are first washed with an isotonic buffer to remove extracellular contaminants and then subjected to homogenization. Homogenization involves physically breaking open __________ ___ ____ ____ ___ ___ _________.
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