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Confocal microscopy is based on the principle of optical sectioning, which allows the observation of a specific focal plane within a specimen while excluding out-of-focus light. This is achieved using a spatial pinhole placed in front of the detector, which blocks light coming from planes above or below the focal plane. A laser beam scans the specimen point by point, and the emitted fluorescence ________ ____ _______ ____ _______ _____.
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