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Km, or the Michaelis constant, represents the substrate concentration at which an enzyme achieves half of its maximum reaction velocity (Vmax). It is a measure of the affinity of the enzyme for its substrate. A low Km value indicates high affinity, meaning the enzyme requires a small amount of substrate to reach half of Vmax. Conversely, ______ ________ _______ __________ _____ _____ _______ ________ ______.
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