Explain the Sanger method of identification of N-terminal of a peptide

Question

22 Jan 2021

Answer :

Word Count : 266

The N terminal amino acid of the protein is labeled by Sanger’s reagent. DNP derivatives are stable to acid hydrolysis. The labeled protein is then hydrolyzed and loaded on chromatography column and the elution profile is matched with the standard profile obtained from FNB ____ ______ _________ _________ ___ ___ ___ __________ _____ ________ _______.
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