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The activity of fatty acid synthase (FAS), a multifunctional enzyme complex responsible for de novo synthesis of long-chain fatty acids, is tightly regulated at multiple levels in animals to meet cellular energy and lipid requirements. One primary regulatory mechanism is allosteric control. Citrate, a key metabolite from the tricarboxylic acid cycle, acts as an allosteric activator of FAS by promoting the polymerization __________ __________ ________ ________ _________ _________ _____ ____ _____ ___ ____ __________.
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