Discuss the protein concentrating technique that is based on freeze drying principle.

Freeze drying, also known as lyophilization, is a technique used to concentrate proteins by removing water from a protein solution without altering the structure of the protein. This method relies on sublimation, where water transitions directly from a solid state (ice) to a gas (vapor) without ___ ________ _______ ________ _______ ______ _______ ____ _____ ___ ________ _____.
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