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Nanopore DNA sequencing is a third-generation sequencing technology that enables the direct analysis of DNA and RNA molecules without the need for extensive amplification or chemical labeling. It is based on the principle of measuring changes in ionic current as individual nucleic acid molecules pass through a nanoscale pore embedded in a synthetic membrane. This technology has transformed genomic research by providing real-time sequencing, ultra-long read lengths, and portability, making it suitable for applications ranging from laboratory research to field-based diagnostics. The central component of nanopore sequencing is the nanopore, a tiny biological or synthetic protein channel that is inserted into an electrically resistant membrane. When an electric potential is applied across the ___ _______ ___ _________ __________ _____ ________ ____ ______ ________.
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