Question

 Describe Cap analysis of gene expression. 

30 Jul 2026
Answer :
Word Count : 625
Cap Analysis of Gene Expression (CAGE) is a high-throughput molecular biology technique used to identify the transcription start sites (TSSs) of genes and to measure promoter activity across the genome. It is an important tool in transcriptomics and functional genomics because it enables researchers to determine where transcription begins and how gene expression is regulated under different biological conditions. CAGE specifically targets the 5′ end of messenger RNA (mRNA), which contains a unique 7-methylguanosine cap structure found in RNA polymerase II transcripts. The fundamental principle of CAGE is based on capturing the capped 5′ ends of full-length mRNA molecules. Since only mature and intact mRNA molecules possess this cap structure, CAGE selectively enriches genuine transcription initiation sites while minimizing artifacts resulting from degraded RNA. This _________ ________ _______ ____ ________.
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