Describe Sanger's di-deoxy method of DNA sequencing.

Sanger’s di-deoxy method of DNA sequencing, also known as Sanger sequencing, is a technique that revolutionized the study of genetics by enabling the determination of nucleotide sequences in DNA molecules. This method was developed by Frederick Sanger and his colleagues in 1977 and remains one of the foundational techniques for DNA sequencing, particularly for small to medium-length DNA fragments. The technique is based on the incorporation of chain-terminating nucleotides, which prevents the elongation of the DNA strand at specific points, allowing researchers to identify the sequence of nucleotides in the DNA sample.

The core of the Sanger sequencing method relies on using four nucleotide bases: adenine (A), ___ ___ _______ _______ ________ ___ ______.
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