Describe isoelectric focusing technique.

Isoelectric focusing (IEF) is a powerful technique used to separate proteins or peptides based on their isoelectric points (pI), which is the pH at which a molecule carries no net charge. The principle behind isoelectric focusing relies on the fact that when proteins are placed in a pH gradient, they migrate through the gel or solution until they reach the point where their net _________ _____ ________ ___ ____.
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