Explain the principle and applications of the following:a) High-Pe

Question

Explain the principle and applications of the following:

a) High-Performance Thin-Layer Chromatography

b) Liquid Chromatography-Mass Spectrometry

23 Jan 2025

Answer :

Word Count : 351

a) High-Performance Thin-Layer Chromatography (HPTLC)
Principle: HPTLC is an advanced form of thin-layer chromatography (TLC) that utilizes a high-quality, uniform thin layer of adsorbent material (typically silica gel or alumina) on a glass, plastic, or aluminum plate. It separates components based on their differential affinities for the stationary phase and their solubility in the mobile phase. _____ ___ ________ ______ _____ __________ ____ __________.
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