Explain the principle and applications of the following:a) High-Pe

Question

Explain the principle and applications of the following:

a) High-Performance Thin-Layer Chromatography

b) Liquid Chromatography-Mass Spectrometry

23 Jan 2025

Answer :

Word Count : 351

a) High-Performance Thin-Layer Chromatography (HPTLC)
Principle: HPTLC is an advanced form of thin-layer chromatography (TLC) that utilizes a high-quality, uniform thin layer of adsorbent material (typically silica gel or alumina) on a glass, plastic, or aluminum plate. It separates components based on their differential affinities for the stationary phase and their solubility in the mobile phase. The separation is achieved through capillary action, where the sample is spotted _________ ____ _________ _______ _______ ___ ____ ________.
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