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Centromere mapping in Neurospora is a classical genetic technique used to determine the distance of a gene from the centromere based on the analysis of ordered asci produced during meiosis. Neurospora crassa is especially suitable for this type of analysis because it forms linear asci containing eight ascospores arranged in a definite order that directly reflects the sequence of meiotic divisions. This ordered arrangement allows precise observation of segregation patterns of alleles, making it possible to locate genes relative to the centromere. During meiosis in Neurospora, a diploid nucleus undergoes meiosis I ______ __________ __________ ___ _________ __________.
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