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pBR322 is one of the most widely used cloning vectors in genetic engineering and biotechnology. It is a small, circular double-stranded plasmid DNA of about 4361 base pairs, originally constructed by Bolivar and Rodriguez. The plasmid contains several important genetic elements that make it suitable for use as a cloning vector. It has two antibiotic resistance genes: the ampicillin _____ _________ _______ _____ ____ ___ _____ ___.
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