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Shelf-life extension of biological materials in both animal and plant tissue culture is critical for maintaining viability, reducing contamination, and enabling large-scale propagation. One primary strategy involves optimizing the culture medium composition. For plant tissues, using nutrient media with appropriate concentrations of sugars, vitamins, and growth regulators can slow metabolic activity, thereby delaying senescence. Similarly, in animal cell culture, reducing the concentration of growth stimulants or _____ _____ _________ __________ _________ _______.
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