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Size exclusion chromatography (SEC), also known as gel filtration chromatography, is a separation technique based on the size and molecular weight of molecules rather than their chemical properties or affinity. One of its unique features is that it separates compounds without relying on interactions such as charge, polarity, or binding affinity. Instead, separation occurs due to differences in the ability of molecules ______ _______ _________ _______ _________ ____ _______.
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