Explain why the atomic spectra are line spectra whereas the molecular spectra are band spectra.
See Answer →Briefly explain the origin of atomic spectrum.
See Answer →What is meant by room temperature phosphorescence? How is it achieved?
See Answer →Describe the application of fluorescence measurements in medical diagnosis by taking the example of blood glucose determination.
See Answer →What is chemiluminescence? Describe an analytical application of chemiluminescence in the area of environmental pollution measurement.
See Answer →Why do we need to modify the instrumental set up for the fluorescence spectrometer to make phosphorescence measurements? Describe the modification.
See Answer →The experimental set up for the fluorescence spectral measurement is different from that for the absorption measurement. Comment
See Answer →What is meant by fluorescence quenching? How does it affect the quantum yield of a fluorescence emission?
See Answer →Explain the origin of fluorescence and phosphorescence spectra in terms of Jablonski diagram.
See Answer →State the ‘Rule of mutual exclusion’. What is its significance?
See Answer →Explain the origin of Raman spectrum in terms of quantum theory of radiation.
See Answer →In what way is the standard addition method of calibration in UV-VIS spectrophotometry better than the standard solution method?
See Answer →What are monochromators? Describe the working of a grating monochromator.
See Answer →State the necessary condition for a molecule to show IR spectrum. Will HCl show IR spectrum?
See Answer →The transmittance of a solution, containing 7.3 mg of an potassium dichromate per 100 cm3 , taken in a cuvette of path length of 1 cm is measured at 455 nm. If the percentage transmittance found to be 6, calculate the molar absorptivity of the oxidizing agent.
See Answer →The Gyan Vani transmission of IGNOU is broadcast at 105.6 MHz in the Delhi region. Compute the energy of a photon corresponding to this frequency.
See Answer →Define electromagnetic radiation in terms of the wave mechanical model.
See Answer →Discuss the experimental aspects of DNA Gel electrophoresis including instruments and reagents used.
See Answer →Briefly explain the following:
i) Reverse Osmosis
ii) Nanofiltration
iii) Ultrafiltration
iv) Microfiltration
v) Dialysis
See Answer →Briefly explain the preparative applications of size exclusion chromatography.
See Answer →