Write the principles of thin layer, gelfitration and affinity chromato

Question

Write the principles of thin layer, gelfitration and affinity chromatography. Explain their applications.

06 Jan 2025

Answer :

Word Count : 656

Thin layer chromatography (TLC), gel filtration chromatography, and affinity chromatography are techniques used for the separation and analysis of proteins, among other biomolecules. Each of these methods operates on different principles based on the unique properties of the substances being separated.

Thin Layer Chromatography (TLC)
TLC is a chromatographic technique used to separate components of a mixture based on differences in their affinity to the stationary phase (typically a solid adsorbent) and their solubility in a mobile phase (solvent). The process involves a thin layer of adsorbent material, usually silica gel or alumina, spread on a flat surface such as a glass or plastic plate.

The principle behind TLC lies in the differential migration of components in the sample based on their interactions with the stationary phase and their solubility in the mobile phase. When a solvent moves up the plate by capillary action, proteins in the sample move at different rates, creating distinct bands. The distance traveled by each component depends __________ ____ _______ _____ _____ ___.
____ ______ ___ __________ _____ ________ ___ ___ ____ _________ _________.
________ _______ _____ ___ _____ ______ _________ ________ ______.
______ ___ ___ ___ ______ _____ ________ _______.
________ ________ _____ _______ _________ __________ ___.
____ __________ ____ ____ ________ ___ ________ ______.
___ ______ _____ ______ _____ ______ ____ ________ _______ ____ __________.
___ ___ _____ ___ ____ _________ ___ __________ ________ _______ _____ _________.
_____ ___ ___ __________ _________ _______.
__________ ______ ________ ___ ______ _________ ____ __________ ________ ____ ___ __________.
___ ____ _______ _______ _______ __________.
_______ _____ __________ ________ __________ ____ __________ ___.
_________ ______ ______ __________ ___.
__________ _________ ____ ______ _______ __________ ________.
______ __________ ______ ________ _________ __________ _________.
__________ _____ _________ ___ ___ ___ ______.
_________ ___ _______ ____ _____ _______ _____ __________ ___ _______.
_____ _______ _____ ______ __________ ____ ________ ____.
__________ _____ ________ __________ ________ _____ __________ _______ ________ __________.
_________ ________ ____ ______ ___ ______ _____ _________ ________ _____ __________.
__________ _______ ________ _________ ________ _______ _____ _______.
________ ________ ___ _____ __________ ___ _________ __________ ____ ___.
__________ ________ ____ ____ ___ ____ ______ _____ _____ _________ ___.
__________ _______ _____ ___ ___ _____ _________ ____ ________ _________ ______.
_____ _______ _____ _______ _________ ______ ______ ___ ________ _________.
_______ _______ ____ ____ _____.
__________ _________ _____ ____ _____ ______ _______.
____ ___ ____ ________ _______ _____ __________.
__________ ________ _______ ____ __________ __________.
_____ _______ ______ __________ ________ ___ _____ _________.
__________ ________ _______ _______ ________ ___ ____ ______ _________ ________ __________.
_______ _________ ________ ___ ____.
____ ________ _______ __________ _______ _______ ________ ____ __________ _____ __________ __________.
______ _____ _________ ___ ___ ___ _____.
___ ______ _____ _______ __________.
________ _________ ________ ____ ________ _____ _________.
_______ __________ _______ _____ ____ _______ ________.
______ _____ ___ ________ _____ _________.
______ __________ ____ ________ ___ __________.
_________ _______ ____ ________ ___ ___ ______ _______.
_____ ________ ____ ____ _____ ______ ________ ________ _________.
_____ ___ _______ ______ _____.
____ _________ __________ ______ ______ ____ ____ _______ ____ ___ __________.
_____ ___ ______ ___ __________ _____ ______ ________ ________ ______ _____ ____.
_________ _______ ______ ____ ______ ________ _______.
________ _______ ______ _____ _____ _____.
___ ______ ________ ______ _____ ________ ________ ____ __________ ____ ______.
___ _________ _________ ___ ____ _____ ______ ______ ________ __________ _____ _________.
_____ __________ ____ ___ __________ _____ ___ __________ ______ ___.
___ _______ ____ _______ ______.
__________ _______ _____ _________ _______ ______ ____ ________ _______ ____.
______ _____ _____ ___ _______ ________ __________.
____ ______ ______ _________ _________ __________ ______ __________ _______ ______ ________ _____.
_________ _____ _______ ____ ______ ________ __________ _______.
_____ _________ _________ _______ ___.
________ _________ _______ __________ ______ __________ ___ ________ __________ ______.
__________ _______ _______ _______ __________ _______ _______ _____.
_________ _______ __________ __________ ____ _________ _________.
________ _____ ____ _______ _____ ______ ______ _________.
____ ___ ______.
Get Full Answer on WhatsApp

IGNOU Solved Assignments Assignments Ignou Projects Project Previous Year Solved Exam Papers Notes Notes Sample Question Papers Sample Question Papers

IGNOU NEWS

Assignment Submission Last Date Extended Till 30 June 2026 Click Here★★★IGNOU June 2026 TEE Date Sheet Released Click Here★★★