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Electrophoresis is a separation technique that is based on the principle of movement of charged molecules under the influence of an electric field. Proteins, being amphoteric in nature, carry both positive and negative charges depending on the pH of the medium. When an electric field is applied, the net charge on a protein determines the direction and rate of its migration. The mobility of a molecule depends on its charge-to-mass ratio, shape, and the strength of the electric field. The medium, such as a gel or capillary, provides resistance and acts as a supporting matrix to facilitate separation of proteins based on their different electrophoretic mobilities. This principle allows the resolution of complex mixtures of proteins into distinct ______ _____ ________ _____ ________ __________.
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