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Electrophoresis is a separation technique that is based on the principle of movement of charged molecules under the influence of an electric field. Proteins, being amphoteric in nature, carry both positive and negative charges depending on the pH of the medium. When an electric field is applied, the net charge on a protein determines the direction and rate of its migration. The mobility of a molecule depends on its charge-to-mass ratio, shape, and the strength of the electric __________ _______ ________ ________ ____.
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