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Fluorescence spectrophotometry is a sensitive analytical technique used to detect and quantify molecules that emit light upon excitation. The basic components of a fluorescence spectrophotometer include a light source, typically a xenon or mercury lamp, which provides high-intensity light to excite the sample molecules. An excitation monochromator or filter selects the specific wavelength of light that matches the absorption spectrum of the analyte. __________ _______ _________ ________ ________ ____ _______.
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