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Phagemids are hybrid vectors that combine features of plasmids and bacteriophages, primarily designed to facilitate the cloning and manipulation of DNA in molecular biology. They contain an origin of replication from a plasmid, allowing them to replicate independently within a bacterial host, as well as sequences derived from filamentous bacteriophages, such as M13, enabling them to package single-stranded DNA into phage particles. This dual functionality makes phagemids highly versatile tools for __________ _____ __________ ___ _______ _________.
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