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Recombinant DNA technology involves the manipulation and combination of DNA from different sources to create new genetic combinations. One of the fundamental tools in this technology is the plasmid vector, which plays a critical role in cloning and gene manipulation. A plasmid vector is a small, circular, double-stranded DNA molecule found naturally in bacteria, separate from their chromosomal DNA. These plasmids are capable of autonomous replication inside bacterial cells, making them ideal carriers or vectors for foreign DNA in genetic engineering. The construction and development of a plasmid vector begin with selecting an appropriate plasmid _____ _______ _____ ____ _______ ______ _______ _____.
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