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Plant tissue culture is a technique used to grow plants under sterile conditions on a nutrient culture medium. The main steps include explant selection, sterilization, initiation of culture, multiplication, rooting, and acclimatization. An explant, which is a small piece of plant tissue such as a leaf, stem, or root, is taken and sterilized to _____ _____ _______ ______ _______ _____ _____ ______ _____.
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