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In biological systems, many reactions that are essential for life are thermodynamically unfavorable, meaning they have a positive Gibbs free energy change (ΔG). To drive such reactions forward, cells employ the principle of coupling, where an energetically unfavorable reaction is linked with a highly favorable one. This process ensures that overall ΔG becomes negative, making the combined reaction spontaneous. The most _________ __________ ___ _______ _________ _______ ____ ___ ___.
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