What is the principle of paper chromatography? How is the technique of TLC helpful in the identification of a compound?
Paper chromatography is a widely used analytical technique for separating and identifying the components of a mixture based on their differential migration through a stationary phase (usually a piece of filter paper) caused by their varying affinities for a mobile phase (usually a solvent). The principle of paper chromatography is based on the concept of partition chromatography, which relies on the distribution of a solute between two phases: a stationary phase (paper) and a mobile phase (solvent).
Here's how paper chromatography works:
-
Sample Application: A small spot of the mixture to be analyzed is placed near the bottom of a strip of filter paper or chromatography paper.
-
Development: The bottom of the paper strip is then immersed in a solvent (the mobile phase). As the solvent travels up the _________ ________ ______ ________ ___ _______ _______ ______ ______.
___ ___ _________ ___ _________ _______ __________.
_______ ______ ____ ____ ______ ____ ________ _________ ______ ____ _______ ___.
______ ___ _____ _________ _____ ________ ____ ______.
__________ _______ _________ __________ _________ _______ ____ _________.
___ ________ ___ _________ ___ _____ _____ __________ __________ ____ ______.
_________ ____ ______ ____ ___ _________ __________ ____ _______ ___ ___ _________.
___ _____ ________ ________ _______ ____ __________ ___ ____ ________.
__________ _____ ____ __________ ________ ____ _______ ________.
_____ ____ ___ ________ _________ _________ ______ ____ _________.
____ _________ ______ _________ ____ ___ _______ ______.
______ ___ ______ _____ ___ __________.
________ _______ __________ ________ __________ ___ __________ _______ ___ _________ ________.
________ ________ _____ ________ __________ __________ ______ ______ _____ ________.
_____ _________ _________ _________ _________ ______ ________ ______ _______.
_________ _________ _________ ____ ___ _____.
____ _________ _______ ______ ______ ________ __________ ____ ___ ________ ___ _________.
_____ ___ __________ ________ __________ _______ ______ ______ __________ ___.
_________ ________ _______ ____ ___ ____ ________ _____.
____ ____ __________ ______ ________ ________ ________ _______ ____ __________.
___ _______ ______ ______ ____ ______ _____ ______ ___.
_____ _____ ______ _________ ____ _____ _________ ___ ________ _______ ___ ______.
________ ____ __________ __________ ________ _____ _________ __________ ___.
_______ __________ __________ _____ _________ _____.
________ _______ ____ ____ ___ __________ _________ _______ ________ _________.
__________ ___ ____ _________ ___ ___ __________ _________.
_______ _____ ________ _______ _______ __________ ____.
__________ _________ ______ ______ __________ ___ ______.
___ _____ ________ ____ ________ ______.
________ ___ _______ ___ _________ _________ _______ __________ _______.
_______ ___ ___ ______ ____ ____ ________ ___ _______ ___.
___ ____ ___ _____ _______ __________ __________ __________ __________.
_________ ______ _________ _______ ________ _________ ____ ______ ______ ___.
_____ ______ __________ _______ __________ __________.
___ __________ ________ _______ ______ _________ ___.
__________ __________ __________ _______ _____ _________ ________ ________.
_____ _______ __________ _____ _________ ________ ___ _____ __________ _________.
_____ _________ ___ ________ _______ ________ ________ ________ __________ _____ ________.
__________ _______ _______ ___ _______ __________ ___ __________.
_____ __________ _____ _______ _______.
______ _______ _______ _________ ___ _______.
_______ _____ ___ ___ _____ ____ ____ ___.
_________ ____ __________ _________ ____ ________ ________.
___ ____ ____ _________ ______ _____ __________ _________ _______.
______ __________ ______ __________ _____ ___ _____ ________.
_____ __________ ___ ________ _______.
_______ ______ __________ ______ ____ _________.
Get Full Answer on WhatsApp