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Pyrosequencing is a modern DNA sequencing technique based on the detection of pyrophosphate release during nucleotide incorporation. Unlike traditional Sanger sequencing, which relies on chain termination, pyrosequencing uses a sequencing-by-synthesis approach, allowing real-time monitoring of DNA synthesis. In this method, a single-stranded DNA template is hybridized with a sequencing primer, and DNA polymerase catalyzes the addition of complementary nucleotides one _____ ________ _______ __________ _______ _______ ______ _____ _________.
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