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The determination of amino acid sequence in a protein is essential for understanding its structure and function. The main methods include Edman degradation, mass spectrometry-based sequencing, enzymatic or chemical cleavage followed by sequence analysis, and gene-based sequencing from the corresponding DNA or mRNA. Edman degradation is a classical method, while mass spectrometry methods such as MALDI-TOF and ESI-MS ___ ________ ___ ________ ___ _________ __________ ________ __________ __________.
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