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Ligases are a class of enzymes that catalyze the joining of two separate molecules by forming a new covalent bond. This process is usually coupled with the hydrolysis of a high-energy phosphate bond from adenosine triphosphate (ATP) or a similar nucleoside triphosphate. Because of this energy requirement, ligases are also known as synthetases. They play an essential _____ _____ ____ ______ __________ _____ ___ ___ _____.
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