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DNA ligase plays a crucial role in joining DNA fragments during molecular cloning, DNA replication, and repair processes. It catalyzes the formation of phosphodiester bonds between the 3' hydroxyl group of one DNA strand and the 5' phosphate group of another. This enzymatic action effectively seals the "nicks" in the ________ ____ ___ __________ _________ ________ _______ _______ ___ __________ ________.
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