Explain the N-terminal protein sequencing method

The N-terminal protein sequencing method, also known as Edman degradation, is a technique used to determine the amino acid sequence of proteins from the N-terminus. This method was developed by Pehr Edman in 1950 and remains a standard technique for sequencing short peptides and proteins.

The process begins with the isolation of the protein of interest, which is then subjected to mild conditions to _____ ____ ________ _____ ___ ____.
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