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Recombinant insulin is produced using genetic engineering techniques where the gene encoding for human insulin is inserted into a suitable host, typically *Escherichia coli* or *Saccharomyces cerevisiae*. The gene is first isolated and inserted into a plasmid vector, which is then introduced into the host cell. Once inside the cell, _____ _________ _________ ___ ______ __________ ________ _____ ___.
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