Discuss the methods for the separation of macromolecules based on their size, charge, solubility and molecular affinity

The methods for separating macromolecules on the basis of:

molecular size: gel-filtration chromatography (proteins are less soluble in solutions containing high salt than they are in low-salt solutions; so that a mixture of proteins can potentially be separated by addition of large quantities of a salt). The denslty gradient in the solution is created by adding theconcentrated and highly soluble materials, for example, sucrose for protein and ceasium chloride for nucleic acids. This gradient helps in the formation and stabilisation of distinct bands ___ ____ ___ ________ _____ ____ __________.
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