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DNA microarray analysis involves a systematic sequence of steps designed to measure the expression levels of thousands of genes simultaneously and to understand global patterns in cellular function. The process begins with the isolation of high-quality RNA from the biological samples being compared, such as diseased versus healthy tissues or treated versus untreated cells. Since microarrays measure gene expression at the transcript level, it is essential that the extracted RNA is intact, free from contaminants, and representative of the cellular transcriptome. The RNA is then converted into complementary DNA (cDNA) or complementary _________ ________ ____ __________ ______ ______.
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