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Two-Dimensional (2D) Gel Electrophoresis is a powerful technique used in proteomics to separate complex mixtures of proteins based on two distinct properties: isoelectric point (pI) and molecular weight. The principle involves two sequential steps. In the first dimension, proteins are separated by isoelectric focusing (IEF), where an electric field is applied ____ _________ ________ __________ ________ ___ ____ ____.
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