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Glycolysis is a central metabolic pathway occurring in the cytosol of all living cells in which one molecule of glucose is enzymatically broken down into two molecules of pyruvate. This pathway is divided into two major phases: the preparatory phase and the energy generation phase. The preparatory phase is also called the energy investment phase because it consumes ATP to activate glucose, while the energy generation phase is responsible for ATP and NADH production through the breakdown of three-carbon intermediates. In the preparatory phase, glucose first enters the cell and is phosphorylated by hexokinase or glucokinase to form glucose-6-phosphate, using one ATP molecule. This step ____ ____ ___ _____ _________.
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