Which different factors make the results of TLC difficult to reproduce?

Thin Layer Chromatography (TLC) is a widely used analytical technique, but several factors can complicate the reproducibility of results. One of the most significant factors is the variation in the preparation of the TLC plate. The uniformity of the stationary phase (usually silica gel) is crucial for consistent results, and any _______ ______ ______ ______ _____ _________ ___ __________ _________.
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